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. 2021 Jun 4;29(10):2949–2962. doi: 10.1016/j.ymthe.2021.06.001

Figure 1.

Figure 1

Biochemical characteristics of HCW9218

(A) Cartoon models of the heterodimeric bifunctional fusion protein complex, HCW9218, comprising 2∗TGFβRII-TF-IL-15 and 2∗TGFβRII-IL-15RαSu polypeptides. (B) The polypeptides of HCW9218 are highly glycosylated. To examine the molecular weight characteristics, we ran deglycosylated and untreated protein samples on 4%–12% SDS-PAGE Bis-Tris gels under reducing conditions and stained with InstantBlue. Lane 1, non-deglycosylated HCW9218; Lane 2, deglycosylated HCW9218; Lane M, Mark12 unstained molecular weight markers. (C) High-performance liquid chromatography (HPLC)-SEC analysis of purified native and deglycosylated HCW9218 samples. Characteristics of purified HCW9218 generated in an engineering run (50 L) scale process are shown in Table S1 (also see Figure S1).