FIG. 10.

TBP mutants interact normally with the N-terminal inhibitory domain of TAF130 and the general negative regulator NC2. (A) GST fusions to regions of TAF130 that contain (10–88) or lack (10–58) the N-terminal inhibitory region were incubated with the indicated TBP derivatives. After GST pulldown, the associated proteins were analyzed by Western blotting with TBP antibodies. (B) The indicated amounts of recombinant NC2 were incubated with 200 ng of histidine-tagged TBP or TBP-ΔN69 in the presence of Ni-NTA-agarose. The amount of NC2 retained by the TBP derivatives was analyzed by Western blotting using antibodies to NC2. wt, wild type.