FIG. 8.
TBP mutants are defective for TATA element binding in vitro. The indicated amounts of wild-type (wt) and mutant TBP derivatives were incubated with a double-stranded oligonucleotide containing a consensus TATA element from the adenovirus 2 E1B promoter, and TBP-DNA complexes were resolved on a 5% Tris-glycine-EDTA polyacrylamide gel containing 4 mM MgCl2.