TABLE 3.
Effects of vitamin B6 metabolite, pyridoxal 5′-phosphate (PLP), on cardiac function and sarcoplasmic reticular (SR) Ca2+-transport in rat hearts subjected to ischemia-reperfusion (I/R).
| Parameters | Control | Untreated I/R | 25 μM PLP treated I/R |
| A. Cardiac function | |||
| LVDP, mm Hg | 118 ± 5.4 | 39 ± 2.1* | 96 ± 5.2† |
| LVEDP, mm Hg | 7.8 ± 0.6 | 62 ± 3.7* | 25 ± 3.1† |
| +dP/dt, mm Hg/s | 2680 ± 134 | 488 ± 16.7* | 2278 ± 87† |
| –dP/dt, mm Hg/s | 2346 ± 125 | 396 ± 15.4* | 2042 ± 7.4† |
| B. SR Ca2+-transport | |||
| Ca2+ uptake, nmoles Ca2+/mg/min | 28.6 ± 0.77 | 7.9 ± 0.46* | 20.4 ± 0.65† |
| Ca2+ release, nmoles/mg/15 s | 7.2 ± 0.24 | 2.3 ± 0.18* | 5.9 ± 0.23† |
I/R in isolated perfused rat hearts were induced by 30 min of global ischemia followed by 30 min of reperfusion. PLP was present in the reperfusion medium 10 min before the induction of I/R and was also present throughout the reperfusion period. The data are taken from our paper (Dhalla et al., 2013). *P < 0.05 vs. control group. †P < 0.05 vs. untreated group.