FIGURE 2.

Nlgn1^–/– synapses display a change in the balance of exocytosis and endocytosis. Nlgn1^–/– and WT neurons transfected with sypHy were stimulated with 300 action potentials at 10 Hz, and change in fluorescence monitored over time. Neurons were then perfused with NH₄Cl to reveal total sypHy fluorescence. (A) Overview of experimental paradigm. (B) Representative images of relative sypHy fluorescence at baseline, following 300 action potentials (end 300 AP), 120 s after cessation of stimulus train (recovery), and following perfusion with NH₄Cl. Scale bar indicates 5 μm. Images are false coloured to show relative sypHy fluorescence, with warmer colours indicating increased fluorescence intensity. (C) Time trace of mean (± SEM) changes in sypHy fluorescence, normalised to total fluorescence. (D) Peak sypHy fluorescence during stimulus train, normalised to total fluorescence. Scatter plots show individual n, bars indicate mean ± SEM. Forest plot shows estimated difference between group means with 95% CI, unpaired t-test, *p < 0.05 (p = 0.0055).N = 14–15 individual fields of view from independent coverslips.