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. 2021 Oct 21;12(11):979. doi: 10.1038/s41419-021-04261-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, b The t-SNE plot showed the distribution of the immune cells from different clusters supervised by Seurat (a) and different tumor progression stages (b) in the atlas. c A table showed the markers used to annotate immune cells and cell accounts of the corresponding cell type. d Dynamic changes of the proportion of the immune cell types spanning from premalignant to late carcinoma. e–g Dot plots showed the expression of paired cytokine and cytokine receptors in ER^low clusters, immune clusters and sub-clusters of LuE2, which indicated that cytokine Cxcl1 and Cxcl16 were highly expressed in sub-clusters of LuE2 and the corresponding receptors Cxcr2 and Cxcr6 were highly expressed in macrophages and T cells.