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. 2021 Oct 21;11:20761. doi: 10.1038/s41598-021-99652-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HFs express key enzymes of gluconeogenesis. ORS-KC and HFs engage in gluconeogenesis and glycogen shunt. Immunofluorescence staining of anagen and catagen HFs for MCT1 (a), LDHB (b), PCK1 (c) and G6Pase (d). Scale bar 100 μm, representative images of 9 images from 3 individuals. (e) Glycogen in ORS-KC with 5 and 10 mM lactate Mean ±SEM. Paired two-tailed t-test, * p < 0.05. (f) Western Blot of GYS1, PCK1, G6Pase, G6PD, LDHB and LDHA in ORS-KC; after 4 and 24 h in 5- and 10-mM lactate. control GAPDH, arrows representing cropped images, representative N = 3 experiments.