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FIG. 6.

FIG. 6

Detection of SFV by PCR. The sensitivity of PCR detection with set B outer primer pair was determined by amplification of M. dunni DNA (105 cell equivalents) containing different numbers of copies of cloned SFV-2 DNA. The number of copies of cloned DNA and the size of the PCR-amplified fragment are indicated. Primers used for detection of the human β-actin gene, which was used as a control for the presence of DNA in each sample, amplified the fragments of the expected sizes.