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. 2021 Oct 21;11:20766. doi: 10.1038/s41598-021-99960-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact on human cell morphology. (A) Human cells (U87) were treated with a variation of concentrations of SAS, DHA, their covalently bound hybrid (AC254) and their 1:1 mixture. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. (B) Cells (U87) were treated with 5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bars represents 20 µm.