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. 2021 Oct 21;12(11):978. doi: 10.1038/s41419-021-04270-x

Fig. 3. Mitochondrial cytochrome c release in response to plicamycin treatment.

Fig. 3

Wild-type (WT) MDST8 and LoVo cells were treated 25, 50, or 100 nM plicamycin (PLI) or 10 nM, 100 nM, 1 µM everolimus (EVE) for 24 h or 48 h followed by immunofluorescence staining with antibodies specific for cytochrome c and subsequent assessment by confocal microscopy. Representative images of cells in each condition are shown. Scale bars represent 10 μm. A, C Images were quantified of cytoplasmic cytochrome c intensity and are reported as a bar chart (B, D). Error bars indicate SEM. Asterisks refer to significant effects for treatments versus control (paired Student’s t test Error bars indicate SEM. Asterisks refer to significant effects for treatments versus control (paired Student’s t test; **P < 0.01, ***P < 0.001).