Skip to main content
. 2021 Oct 21;11:20763. doi: 10.1038/s41598-021-00325-3

Figure 4.

Figure 4

Canine Tregs constitutively express CTLA4. Activated cPBMCs were first stained with cHcAb6. The cHcAb6 stained PBMCs were fixed, permeabilized, and treated with anti-CD3, CD4, CD8, and FoxP3 antibodies. The bound cHcAb6 was detected using anti-canine IgG Fc-750 Ab. Canine IgG was used as an isotype control. (A) The CTLA4 was predominantly expressed on helper T cells and a small subset of cytotoxic T cells. (B) CTLA4 was constitutively expressed on Tregs. MFI Mean fluorescence intensity.