TABLE 2.

Comparison of bright-field and phase-contrast microscopy, culture growth, and FISH evaluations of corneal scrapings as follow-up to clinical presumption of AK

Scraping code no.a	Date of scraping (mo/day/yr)	Clinical diagnosisb	Acanthamoebae detected by microscopy of wet filmsc	Acanthamoebae cultured from scrapingsd	Acanthamoebae detected by GSP
1ae	06/06/95	Typical AK	−	−/+	−
1be	06/07/95	Typical AK	+	+/+	+
6	06/27/95	Typical AK	+	+/+	+
4	07/06/95	Typical AK	−	+/+	+
12	07/10/95	Typical AK	+	+/+	+
2	09/13/95	Typical AK	−	+/+	+p
13	10/02/95	Typical AK	+	+/+	+
9	10/17/95	Typical AK	+	+/+	+
10	02/05/96	Typical AKf	−	+/+	+q
19	?	Atypical AKg	−	+/+	+
18	12/03/94	Atypical AKh	−	+/+	+
8	09/18/95	Atypical AKi	−	−/+	+p
5	10/12/95	Atypical AK	−	+/+	+
15a	08/12/95	Typical AKj	ND	−/−	−
7	09/07/95	Typical AKk	−	−/−	−
16a	09/22/95	Typical AKk	ND	−/−	−
23	10/23/95	Typical AK	−	+/−	−
3akl	10/25/95	Typical AK	−	−/−	−
3bkl	10/25/95	Typical AK	−	−/−	−
21	05/09/95	Non-AK BKNm	−	−/−	−
22	10/03/95	Non-AK MKn	−	−/−	−
14	12/14/95	Non-AK Ci	−	−/−	−
11	01/24/96	Non-AK MK	ND	−/−	−
17	05/24/96	Non-AK BKSo	+	−/+	−

^aAll samples except no. 15 and 16 were fresh corneal scrapings transferred to sterile isotonic saline. The exceptions are scrapings originally spread on non-nutrient agar plates and then recovered from plate washings. 

^bC, conjunctivitis; MK, presumed microbial keratitis of bacterial origin; BKN, bacterial keratitis due to Neisseria meningitidis; BKS, bacterial keratitis due to Staphylococcus aureus. 

^cBright-field or phase-contrast microscopy of original wet films. +, acanthamoebae detected; −, acanthamoebae not detected; ND, not done. 

^dCulture results at TIO/OSU. 

^eScrapings 1a and b were from the same patient. 

^fSevere AK after 3 weeks on chlorhexidine therapy. 

^gTrachoma and acanthamoebae in a non-CL wearer (30). 

^hLarge epithelial defect, sporadic diffuse corneal endotheliitis thought to be due to herpes simplex virus in a non-CL wearer. 

ⁱCL-associated conjunctival inflammation without keratitis. 

^jPatient was on Brolene and neomycin therapy at time of scraping; acanthamoebae found in CL storage case. 

^kPatient was on topical 0.02% chlorhexidine therapy at time of scraping; acanthamoebae found in CL storage case. 

^lBoth eyes of same patient; acanthamoebae found in CL storage case. 

^mBasement membrane dystrophy. 

ⁿRed eye, pain, and photophobia in a CL wearer. 

^oPeripheral ulcer in a CL wearer due to S. aureus, with transient acanthamoebae in the scraping sample (28). 

^pOne cyst seen. 

^qTwo cysts seen.