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. 2021 Oct 8;14:716675. doi: 10.3389/fnmol.2021.716675

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Copyright © 2021 Tse, Nath, Larosa and Wong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chronic stress reduces exNMDAR functions. (A) Left: Histograms of normalized weight gain of control (n = 7) and chronically stressed rats (n = 8) in 21 days. Right: Histograms of CORT levels in trunk blood in control (n = 7) and chronically stressed rats (n = 8) collected right before brain slice preparation. Stressed rats were sacrificed 7 days within the end of chronic restraint stress. Mean ± SEM. *p < 0.05, **p < 0.01, Student’s t-test. (B) Left: Representative traces of evoked NMDAR-mediated field excitatory postsynaptic potential (fEPSP) that were recorded from brain slices of control and chronically stressed rats. Scale bar = 0.2 mV, 40 ms. Right: Scatter plots show the relationship between fiber volley amplitude and the slope of NMDAR fEPSP in control (7 recordings, 4 rats) and stressed rats (8 recordings, 4 rats). Mean ± SEM. (C) Left: Representative traces of evoked AMPAR-mediated fEPSP that were recorded from brain slices of control and chronically stressed rats. Scale bar = 0.4 mV, 25 ms. Right: Scatter plots show the relationship between fiber volley amplitude and the slope of AMPAR fEPSP in control (10 recordings, 4 rats) and stressed rats (13 recordings, 4 rats). Mean ± SEM. (D) Left: Representative traces of sNMDAR and TBOA-induced exNMDAR currents from brain slices of control or chronically stressed rats. Scale bar = 50 pA, 500 ms. Right: Histogram shows the normalized amplitude of exNMDAR currents recorded from brain slices of control (5 recordings, 4 rats) and chronically stressed rats (7 recordings, 6 rats). Mean ± SEM. *p < 0.05, Student’s t-test. (E) Left: Representative traces of APV (50 μM) -induced blockade of whole-cell current of CA1 neurons in brain slices from control and chronically stressed mice. Scale bar = 40 pA, 1 min. Right: Histograms of the APV-induced blockade of whole-cell currents recorded from control (6 recordings, 3 rats) and chronically stressed mice (6 recordings, 3 rats). Mean ± SEM. *p < 0.05, Student’s t-test.