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Orthogonal partial least squares discriminant analysis (OPLS-DA) plot of rectal metabolites in comparisons of the LE and HE groups following (A) positive ion electrospray ionization (ESI+) and (B) negative ion electrospray ionization (ESI–). (C) Identification of the differentially abundant metabolites between the LE and HE groups. Red represents an upregulation, while green represents downregulation; blue represents no change. (D) Enrichment analysis of metabolic pathways.
