TABLE 1.

Multivariate analysis of metabolites in wild and captive-reared S. o’connori.

Multivariate analysis	Group	Positive ion model						Negative ion model
		A	R2X	R2Y	Q2	R2 intercept	Q2 intercept	A	R2X	R2Y	Q2	R2 intercept	Q2 intercept
PCA	QC	3	0.507		0.219			3	0.532		0.243
	OD vs OW	2	0.503		0.0346			2	0.529		0.0701
	LD vs LW	2	0.495		−0.043			2	0.542		0.0148
PLS-DA	OD vs OW	2	0.5	0.998	0.852			2	0.521	0.997	0.924
	LD vs LW	2	0.469	0.999	0.907			2	0.533	0.999	0.942
OPLS-DA	OD vs OW	1 + 3 + 0	0.714	1	0.943	1	0.614	1 + 4 + 0	0.82	1	0.958	1	0.787
	LD vs LW	1 + 3 + 0	0.725	1	0.921	1	0.633	1 + 3 + 0	0.752	1	0.961	1	0.53

PCA, principal component analysis; PLS-DA, partial least squares-discriminant analysis; OPLS-DA, orthogonal partial least squares-discriminant analysis; QC, quality control; LW, liver from wild S. o’connori; LD, liver from domestic S. o’connori; OW, oocyte from wild S. o’connori; OD, oocyte from domestic S. o’connori.