Table 1.
Polymerase chain reaction primers for polymerase chain reaction off-target assay
| Primers - off targets | |
|---|---|
|
| |
| Name | Sequence (5`-3`) |
| OT1F | TGGTTGCTTTACATCTTCTG |
| OT1R | GAGTCATTGAGCTAGGTCTG |
| OT2F | GTGTCAAGAAGGAGAGGCGC |
| OT2R | GCAAAGCTGAGGTGATGGGG |
| OT3F | GCTTTCTGATCACACCCAAG |
| OT3R | TAGCATTATCTGGCCTGCCC |
| OT4F | ATTGCGCTCTCTTCATTCGG |
| OT4R | AGAGAGGGGAACTGACAAGC |
| OT5F | CACTTGTGTGGATGTAAAAG |
| OT5R | ATCTCTTATGTGCAGGCATC |
| OT6F | AGGGAGAATTTTGTTGCTTG |
| OT6R | TCACTGTGAACACCTGAGAG |
| OT7F | TTGGTGTGGGGCAGTGACTG |
| OT7R | TTCTGCGCCAAGGGAAATGG |
| OT8F | ATTGAACTCACAGAGATCTG |
| OT8R | GGACTAGTATATTGTCTTCCTC |