FIG. 1.

Blankophor staining and subsequent immunofluorescence for candidiasis. (A) A section of the liver of a leukemic patient who had succumbed to culture-proven C. albicans mycosis during neutropenia was deparaffinized and stained with Blankophor (1,000-fold diluted stock solution in saline) which proved the mycotic origin of the fatal disease. (B) Subsequently, indirect immunofluorescence for C. albicans was carried out on the same site in the same tissue section, using polyclonal rabbit antibodies against C. albicans (code B143; DAKO, Copenhagen, Denmark) and a suitable fluorescein-labeled second antibody. The bar corresponds to 25 μm.