FIG. 2.

Blankophor staining and subsequent immunofluorescence for zygomycosis on a section of the lung of a neutropenic patient who had succumbed to double mycosis caused by culture-proven A. fumigatus and a noncultured zygomycete. (A) After deparaffinization, Blankophor in saline was applied as described in the legend for Fig. 1. (B) Subsequently, indirect immunofluorescence for zygomycosis was performed essentially as described in the legend for Fig. 1, using murine monoclonal antibodies against zygomycetes (code M3565; DAKO) and a suitable fluorescein-labeled second antibody. Comparison of panels A and B reveals the presence of elements of a second fungus at the same site. The bar corresponds to 25 μm.