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. 2021 Oct 8;12:703011. doi: 10.3389/fpls.2021.703011

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Copyright © 2021 Cui, Ge, Zhao, Chen, Sang, Zhao, Chen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of GhDRP silencing on V. dahliae resistance of cotton. (A) Phenotypes of TRV::GhDRP and TRV::00 plants induced by V. dahliae at 25 dpi. (B) Phenotype of positive control. (C) Transcript levels of GhDRP in the leaves of TRV::GhDRP and TRV::00 plants. CK1 and CK2 represent the control plants TRV::00; L1 and L2 represent the gene silencing plants TRV::GhDRP. Asterisk indicates statistically significant differences determined by Student’s t-test (^**p<0.01), and the same below. (D) DI in TRV::GhDRP and TRV::00 plants at 20 dpi and 25 dpi. (E) Relative amount of fungal DNA in the leaves of TRV::GhDRP and TRV::00 plants at 20 dpi and 25 dpi. (F) The fungi in the stem segment were restored on PDA culture medium. (G) Trypan blue staining of the leaves of TRV::GhDRP and TRV::00 plants at 25 dpi. (H) Cell morphology of the stem cross section of TRV::GhDRP and TRV::00 plants at 25 dpi.