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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: FEBS Lett. 2021 Aug 17;595(18):2383–2394. doi: 10.1002/1873-3468.14172

Figure 3. Differential effects of Hsp104 on substrates containing the NBD2* moiety residing in the cytosol versus ER lumen.

Figure 3.

Wild-type (HSP104) and hsp104Δ yeast were transformed with plasmids engineered for the expression of (A) SZ*, (B) NBD2* (C) Chimera N*, and (D) Ste6p*. A cycloheximide chase analysis was performed following a 30 min temperature shift to 37°C. Data represent the means ± S.E., n = 3–7. All p values determined using Student’s t-test vs the wild-type control, * denotes p<0.05.