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. 2021 Oct 8;12:753435. doi: 10.3389/fimmu.2021.753435

Figure 3.

Figure 3

Key performance indicators of the saliva ELISA. (A) Inter-assay precision was estimated by measuring 8 samples in triplicates on 3 different days. Samples were selected to represent a range of IgG concentrations from high to negative. Results are shown as boxplots (median with interquartile range, whiskers represent 1.5x interquartile range). (B) The coefficient of variation of the inter-assay was calculated for each of the 8 samples. (C) Intra-assay precision was estimated by measuring a positive sample in 12 replicates with 8 3-fold dilutions and represented as boxplots. (D) Coefficient of variation of the intra-assay was calculated for each of the 8 dilutions.