FIGURE 2.

Administration of β3‐AR‐selective blocker SR59230A lowers cAMP levels and partially ameliorates the cystic phenotype in Pdk1^fl/fl; Pax8^rtTA; TetO‐Cre mice. (a) LC‐MS/MS analysis of SR59230A concentration in plasma and kidney tissue samples from wt male mice. (b) Measurement of whole‐kidney cAMP levels in homogenates from cystic mice shows that β3‐AR‐selective blockade lowers cAMP in SR‐treated mice as compared to vehicle‐treated littermates. **p < 0.01, *p < 0.05, ns, not significant. (c) Left to right, representative H&E images from non‐induced (left image) and induced mice (mid and right images, respectively), showing the presence of cysts in the parenchyma from cystic animals. Boxed regions are enlarged (5×) in the panel next to the kidney image. Scale bar = 1 mm. (d) Dot plots showing kidney/body weight ratio, plasma blood urea nitrogen (BUN) and NGAL protein levels normalized to actin as measured upon sacrifice at 4 weeks of treatment. The animals with the lowest levels of blood urea nitrogen are indicated with red dots in the central (blood urea nitrogen) as well as in the right‐most (NGAL/actin) panels **p < 0.01