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. 2021 Sep 26;47:102150. doi: 10.1016/j.redox.2021.102150

Table 3.

Primers used for analysis of human gene expression by real-time PCR.


target gene
primer sequence (5'→ 3′)
POLR2A sense ACCTGCGGTCCACGTTGTGT
antisense CCACCATTTCCCCGGGATGCG
TBP sense CGCCGGCTGTTTAACTTCG
antisense AGAGCATCTCCAGCACACTC
eNOS (NOS3) sense GAACCTGTGTGACCCTCACC
antisense TGGCTAGCTGGTAACTGTGC
NRF2 sense CCCAATTCAGCCAGCCCAGC
antisense AACGGGAATGTCTGCGCCAA
HMOX1 sense CGGATGGAGCGTCCGCAACC
antisense TCACCAGCTTGAAGCCGTCTCG
NQO1 sense CCCCGGACTGCACCAGAGC
antisense CTGCAGCAGCCTCCTTCATGGC
ICAM1 sense ACCATGGAGCCAATTTCTCG
antisense GCGCCGGAAAGCTGTAGATG
CCL2 sense CTCTCGCCTCCAGCATGAAA
antisense AGGTGACTGGGGCATTGATT
VCAM1 sense TGTGCCCACAGTAAGGCAGGC
antisense AGCTGGTAGACCCTCGCTGGA
SELE sense AGCCCAGAGCCTTCAGTGTA
antisense CTCCAATAGGGGAATGAGCA

Abbreviations: POLR2A: RNA polymerase II subunit A; TBP: TATA-box binding protein; eNOS (NOS3): endothelial NO synthase; NRF2: nuclear factor erythroid 2-related factor 2; HMOX1: heme oxygenase (decycling) 1; NQO1: NAD(P)H quinone dehydrogenase 1; ICAM-1: intercellular adhesion molecule-1; CCL2: chemokine (C-C motif) ligand 2/MCP-1: monocyte-chemoattractant protein-1; VCAM-1: vascular cell adhesion molecule-1; SELE: selectin E.