Figure 1.

Timeline of the transgenerational experiment. The experiment was divided into three phases: broiler breeder (1), egg incubation (2), and grow-out (3). Treatments, challenges, data collection and sample collection are shown. Time indicates the moment when the different handlings were done. Data collection for performance purposes was done during the entire experiment and indicated by the red-dashed arrows. Measurement of the Chick quality (Tona score) and intestinal lesions (Lesion score) was done ones, respectively at d 1 and d 15. The E. maxima (d 7) and C. perfringens (d 14) challenge are part of the necrotic enteritis challenge. Abbreviations: ADFI, average daily feed intake; ADG, average daily gain; EM, egg mass; FCR, feed conversion ratio; G:F, gain to feed ratio; Lay%, laying percentage .