Skip to main content
. 2021 Jul 30;100(11):101413. doi: 10.1016/j.psj.2021.101413

Figure 5.

Figure 5

Effects of maternal treatment on the responsiveness of blood-derived primary monocytes isolated in the next generation (grow-out phase 3). Gene expression levels and NO production were measured after ex vivo stimulation with medium or LPS. Offspring received in vivo at d 14 the C. perfringens infection. (A) Relative mRNA expression levels of IL-1β and iNOS were measured in the monocytes that were collected at the age of 21 d (7 d after the in vivo challenge). Monocytes received ex vivo stimulation with medium or LPS (10 µg/mL) for 24 h. (B) Nitric oxide production of primary monocytes collected at d 14, d 21 and d 35. Monocytes were ex vivo stimulated with medium or LPS (1 µg/mL, 10 µg/mL) or a combination of LPS (10 µg/mL) + IFNy (0.1 µg/mL) for 48 h. (A and B) N = 12 animals per treatment. The box extends from the 25th to 75th percentiles. The line in the middle of the box is plotted at the median and ‘+’ at the mean. Whiskers represents 10 to 90 percentile. Effects are represented as superscripts and were considered to be significant when P ≤ 0.05. Abbreviations: iNOS, inducible nitric oxide synthetase; LPS, lipopolysaccharides; NO, nitric oxide.