Table 1.
Primers used for RT-qPCR.
| Target1 | Sequence2 | Accession no. |
|---|---|---|
| Internal reference genes | ||
| ACTB | F: 5’-GCCCTGGCACCTAGCACAAT-3’ | NM_205518 |
| R: 5’-GCGGTGGACAATGGAGGGT-3’ | ||
| IPO8 | F: 5’-ACCTCCGAGCTAGATCCTGT-3’ | XM_015287054 |
| R: 5’-GGCTCTTCTTCGCCAACTCT-3’ | ||
| GAPDH | F: 5’-ATCCCTGAGCTGAATGGGAAG-3’ | NM_204305 |
| R: 5’-AGCAGCCTTCACTACCCTCT-3’ | ||
| Genes associated with inflammation | ||
| IL-1β | F: 5’-GACATCTTCGACATCAACCAG-3’ | XM_015297469 |
| R: 5’-CCGCTCATCACACACGACAT-3’ | ||
| iNOS | F: 5’-CTACCAGGTGGATGCATGGAA-3’ | NM_204961 |
| R: 5’-ATGACGCCAAGAGTACAGCC-3’ | ||
IL-1β and iNOS were used to measure an effect of maternal treatments on proinflammatory immunity.
1
ACTB, actin beta; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; IPO8, Importin 8; iNOS, inducible nitric oxide synthetase.
2
F, forward; R, Reverse.