Figure 3.

Pirb-deficient CD4⁺T cells fail to induce chronic colitis. (A) Schematic representation of T-cell–transfer protocol. Rag^-/- mice received either 400,000 CD4⁺ CD45RB^hi WT (blue) or Pirb^-/- (red) T cells. (B) Clinical score and (C) percentage body weight change (relative to weight at start of the experiment) of Rag^-/- mice after T-cell injection (WT, n = 13; Pirb^-/-, n = 14). (D) Cell counts of CD4⁺IFNγ⁺ and CD4⁺IL17a⁺ T cells in the mLN of mice (WT, n = 7; Pirb^-/-, n = 5). (E) Representative image of colon histology (H&E staining) from Rag^-/- mice. Top row: 4× magnification; bottom row: 20× magnification. (F) Colon histologic scoring from Rag^-/- mice (WT, n = 10; Pirb^-/-, n = 10). (G) Systemic levels of TNFα, IFNγ, and IL17a in serum of Rag^-/- mice at 5 weeks after T-cell transfer (WT, n = 7; Pirb^-/-, n = 7). Cytokine levels were detected by IVCCA. Data are presented as means ± SEM. Statistical analysis was performed using 2-way analysis of variance (P < .05) followed by the (B and C) Sidak multiple comparison test or (D, F, and G) unpaired t test. ∗P < .05 and ∗∗∗P < .001. Data shown encompass 3 independent experiments. IP, intraperitoneally.