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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Gastroenterology. 2021 Jan 7;160(5):1662–1678.e18. doi: 10.1053/j.gastro.2020.12.062

Figure 1.

Figure 1.

Expression of miR-10b-5p is drastically reduced in KIT+-ICCs in male diabetic KitcopGFP/+;Lepob/ob Mice. (A, B) Body weight and fasting blood glucose levels of KitcopGFP/+;Lep+/+ (+/+) and KitcopGFP/+;Lepob/ob (ob/ob) mice (Two-way ANOVA, n=8). (C) Pearson correlation analysis between miRNA-seq data obtained from colonic and jejunal ICC (CICC and JICC, respectively) isolated and pooled from diabetic ob/ob (n=30) and +/+ mice (n=20). (D) Heat map of the 70 most dynamically regulated miRNAs in CICC and JICC of diabetic ob/ob mice. (E) Expression levels of the ten most prominently reduced miRNAs in CICC and JICC of diabetic ob/ob mice from panel E (black box) obtained by miRNA-seq (n=20–30). (F) Expression of miR10b-5p in gastric ICCs of +/+ and ob/ob mice measured by qPCR (n=3). (G, H) Western blot and quantification of KIT in the jejunum and colon of +/+ and ob/ob mice (n=3). Error bar indicate SEM, unpaired t-test. *p < 0.05, **p < 0.01.