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. Author manuscript; available in PMC: 2021 Oct 22.
Published in final edited form as: Neuron. 2008 Aug 14;59(3):425–438. doi: 10.1016/j.neuron.2008.07.018

Figure 8. Columnar-specific refinement is mediated by cholinergic spontaneous activity in the retina.

Figure 8.

(A, B) RGC projections to the SC of a P10 WT CB2-GFP mouse that received epibatidine (epi) injections into the right eye to disrupt spontaneous retinal activity and saline (control) injections into the left eye, from P4-P10. Normal laminar-specific targeting of GFP+ tOFF-αRGC axons to the lSGS is evident in both SC hemispheres. Columnar segregation is normal in the saline hemisphere but is perturbed in the SC hemisphere contralateral to the epi-injected eye. Scale is 250 μm. (C) Quantification of columnar specificity in the SC hemisphere receiving input from control, saline- or epi-injected retina (epi vs. saline **P<0.001; saline versus control p=0.67; ±SEM; t-test; n=2 mice).