FIGURE 2.

Selective activation of astrocytic hM4Di inhibits LPS‐induced glial activation in the mouse hippocampus. (A) Schematic diagram showing the timeline of experimentation. (B) Immunofluorescence staining and image analysis were performed. Astrocytes were immunohistochemically labeled with GFAP (white), and microglia were labeled with Iba‐1 (red or green). Quantitative analysis of GFAP‐positive astrocytes and Iba‐1‐positive microglia as well as relative GFAP and Iba‐1 immunoreactivity (IR) intensity in the hippocampus are presented in adjacent graphs. Scale bar, 200 μm. Results are expressed as means ± SEM (n = 4). *p < .05 between the indicated groups (one‐way ANOVA with Bonferroni's post hoc test). eYFP, AAV5‐hGFAP‐eYFP; hM4Di‐mCherry, AAV5‐hGFAP‐hM4Di; IR, immunoreactivity; ns, not significant