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. 2021 Oct 8;12:719290. doi: 10.3389/fphar.2021.719290

FIGURE 3.

FIGURE 3

The effect of rAAV6-BMP7 gene therapy on diabetes-induced structural changes. Quantification of picrosirius red-stained LV cross sections. (A) Total interstitial collagen content using brightfield imaging, interstitial type I (B) and type III (C) collagen content using polarised light. (D) Representative images of picrosirius red-stained collagen in LV cross sections (top panel, brightfield; bottom panel, polarised light; scale bar: 50 µm). Quantification in LV cross sections of (E) cardiomyocyte width and (F) cardiomyocyte area in haematoxylin and eosin-stained samples, and (G) TUNEL-positive apoptotic cells. (H) Representative images of haematoxylin and eosin-stained LV sections (top panel) and TUNEL-positive apoptotic cells (bottom panel; scale bar: 50 µm). Data are presented as mean ± SEM. n = 9–20 per group (see individual data points). *p < 0.05, **p < 0.01, ***p < 0.001. Two-way ANOVA followed by Tukey’s post hoc test. AAV, adeno-associated virus; BMP7, bone morphogenetic protein 7; H&E, haematoxylin & eosin; LV, left ventricle.