TABLE 1.
Organ weights and systolic heart function in control and diabetic mice following rAAV6-BMP7 or rAAV6-Null treatment.
| Control | Diabetes | |||
|---|---|---|---|---|
| rAAV6-Null | rAAV6-BMP7 | rAAV6-Null | rAAV6-BMP7 | |
| Organ weights | ||||
| n | 9 | 10 | 19 | 25 |
| Tibial length (mm) | 17.9 ± 0.2 | 17.9 ± 0.1 | 18.0 ± 0.1 | 18.1 ± 0.1 |
| Kidney weight (left; mg) | 215 ± 3 | 239 ± 8 | 250 ± 10 | 251 ± 6 |
| Lungs weight (mg) | 157 ± 6 | 160 ± 4 | 199 ± 8*** | 186 ± 4* |
| Liver weight (g) | 1.56 ± 0.04 | 1.68 ± 0.06 | 2.62 ± 0.12*** | 2.56 ± 0.10*** |
| Gastrocnemius weight (mg) | 190 ± 11 | 180 ± 4 | 199 ± 4 | 195 ± 6 |
| Spleen weight (mg) | 97 ± 2 | 106 ± 4 | 154 ± 5*** | 142 ± 4*** |
| Pancreas weight (mg) | 250 ± 11 | 278 ± 13 | 248 ± 11 | 268 ± 16 |
| Fat pad weights | ||||
| Pericardial fat weight (mg) | 14.9 ± 2.4 | 26.5 ± 6.9 | 40.8 ± 4.8** | 34.7 ± 3.9 |
| Perirenal fat weight (g) | 0.68 ± 0.11 | 0.82 ± 0.12 | 1.49 ± 0.14** | 1.60 ± 0.11** |
| Inguinal fat weight (g) | 0.79 ± 0.15 | 0.89 ± 0.16 | 2.56 ± 0.22*** | 2.55 ± 0.21*** |
| M-mode echocardiography | ||||
| n | 9 | 10 | 17 | 22 |
| Heart rate (bpm) | 310 ± 15 | 345 ± 20 | 361 ± 13 | 372 ± 14 |
| AWd (mm) | 0.70 ± 0.01 | 0.70 ± 0.01 | 0.77 ± 0.02 | 0.77 ± 0.01* |
| PWd (mm) | 0.70 ± 0.01 | 0.71 ± 0.02 | 0.77 ± 0.02 | 0.78 ± 0.01 |
| LVEDD (mm) | 4.11 ± 0.05 | 4.14 ± 0.04 | 4.22 ± 0.04 | 4.21 ± 0.05 |
| LVESD (mm) | 2.85 ± 0.08 | 2.84 ± 0.09 | 2.87 ± 0.05 | 2.89 ± 0.07 |
| Fractional shortening (%) | 31 ± 2 | 31 ± 2 | 32 ± 1 | 31 ± 1 |
Data are presented as mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc test.
*p < 0.05, **p < 0.01, ***p < 0.001 vs. corresponding Control. AWd, anterior wall thickness during diastole; LVEDD, LV end diastolic dimension; PWd, posterior wall thickness during diastole; LVESD, LV end systolic dimension.