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. 2021 Oct 21;12:550. doi: 10.1186/s13287-021-02617-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Flow cytometric analysis of pluripotent stem cells after treatment with the SCD1 inhibitor. A Representative flow cytometry dot plots showing four-cell fractions: viable cells (annexin V⁻/PI⁻), early apoptotic cells (annexin V⁺/PI⁻), late apoptotic cells (annexin V⁺/PI⁺), and necrotic cells (annexin V⁻/PI⁺). The induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) were mock-treated with DMSO (< 0.05%, above) or treated with 25 nmol/L of SCD1 inhibitor (SCDinhib) on Day 0 of differentiation (below) for 4 days. Cells were then harvested and evaluated for apoptosis and necrosis. B Quantification of cells in different groups