Fig. 4.

The expression of endoderm and stemness markers following the inhibition of SCD1 activity during induced differentiation. Cells were treated with SCD1 inhibitor (SCDinhib) alone at Days 0, 1, or 2 (0, 1, and 2) of differentiation or in combination with oleic acid (OA) at Day 0. Undifferentiated cells (Undiff) and cells treated with DMSO (< 0.05%, Mock) were served as background controls. The cells were harvested on Day 4. The gene expression in iPSCs (A) and protein expression (representative Western blot and quantification) (B) of the stemness markers Oct4 and Sox2 and endoderm markers CXCR4 and Sox17 were evaluated in the induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) using quantitative PCR and Western blot, respectively. C Quantification of protein expression in different groups. *p < 0.05, **p < 0.01 versus mock, ^#p < 0.05, ^{# #}p < 0.01 versus the same day in SCDinhib