Fig. 4.

Injection of HF-MSC suppressed AA in C3H/HeJ mice. A Hair loss in C3H/HeJ mice intravenously injection of HF-MSC weekly. At 0 and 6 weeks after injection, the image of mice were photographed. B Compared with injected PBS (n = 4), mice did not continue to lose his hair that injected HF-MSC (n = 5). C H&E showed that HF-MSC-injected mice exhibited more hair follicles in anagen, while the PBS group had dystrophic hairs with lymphocyte infiltration (bar = 100 μm).The skin samples were harvested after 6 weeks treatment. D The number of hair follicle growth in the skin, which was averaged from 6 slides (10×). E–F HF-MSC-injection increased Ki67 expression (green) (bar = 100 μm) (E) and suppressed CD8 expression (red) (bar = 200 μm) (F) in C3H/HeJ skin. Relative fluorescence areas were averaged from 6 to 8 fields. G-K The mRNA transcript levels of caspase1, Ki67, MHC I, TNF-α, IL-6 in C3H/HeJ skin at 6 weeks after injection were examined using qRT-PCR, expression levels of those mRNA were normalized with GAPDH. The results were expressed as the Mean ± SD, *p < 0.05