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. 2021 Oct 21;18:243. doi: 10.1186/s12974-021-02299-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of changes in miR-204-5p expression within the hippocampal DG region of normal and CUS rats. A Schematics of AAV vectors engineered to overexpress miR-204-5p, knock-down miR-204-5p and their corresponding controls. B Experimental paradigm for determining behavioral responses of rats infected with the virus. C Illustration of AAV viral infusion into the DG region. Scale bar is 1 mm. D The infection efficiency of AAV-miR-204-5p sponge and AAV-miR-204. Expression level of miR-204-5p in the DG region infected with AAV-204-5p or AAV-204-5p sponge (N = 10 per group). Behavioral responses in the (E) FST (N = 20 per group), (F) SPT (N = 20 per group) and (G) MWM of rats expressing various viral constructs within the DG region (N = 6 per group). *P < 0.05, **P < 0.01 ***P < 0.001, ****P < 0.0001, control + AAV-control vs control + AAV-miR-204-5p sponge; ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001, ^&&&&P < 0.0001, CUS + AAV-control vs CUS + AAV-miR-204-5p. Data are presented as means ± SEMs. Student t-tests were employed for comparisons between the two groups. One-way ANOVA followed by Tukey’s post-hoc test was used for D. Two-way ANOVA was used for E–F