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. 2021 Oct 22;299:114338. doi: 10.1016/j.jviromet.2021.114338

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© 2021 The Author(s)

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 1 — Comparison of RT-SARS and Allplex cycle thresholds in positive SARS-CoV-2 specimens.

Twenty-nine positive samples by Allplex were retested with RT-SARS showing concordantly positive results (100 % PPA). The mean Ct difference (mean of Allplex target regions minus RT-SARS) was 9.6 Ct which is in the range of the 10 unread cycles of RT-SARS. The positive samples in the figure are presented in ascending order of Ct values by RT-SARS. Additionally, 29 SARS-CoV-2 negative specimens by Allplex retested with RT-SARS were found negative (100 % NPA).