Figure 3.

Treadmill exercise inhibited M1 microglia polarization and promoted microglia polarization toward M2 Phenotype after MCAO. Double immunostaining with Iba1 and CD68 or Arg-1 at 24 h, 4, and 7d among the sham, MCAO and exercise groups was performed in this study. (A,B) representative images of immunostaining with Iba1 and CD68 in the cortex (A) and striatum (B) and the corresponding histogram was shown on the right side. (C,D) representative images of immunostaining with Iba1 and Arg-1 in the cortex (C) and striatum (D) and the number of double-positive cells was shown on the right side. White arrows indicated the double-positive cells. Bar = 50 μm for all images, n = 3 per group. ^#P < 0.05, ^##P < 0.01 vs. sham group; *P < 0.05, **P < 0.01 vs. MCAO group.