Fig. 5.

OLFML3 is a RV-inducible suppressor of type I IFN signaling during RV infection. a Structural organization of OLFML proteins. b RT-qPCR quantification of OLFML3 expression levels in H1-Hela cells in the absence or presence of RV-B14 or RV-A16. Samples are collected at 24 h post infection with a RV MOI of 2. c RT-qPCR quantification of IFIT2, OAS2, and ISG15 expression in WT and OLFML3^−/− cells at 0, 12, and 24 h after RV-B14 infection at an MOI of 2. d RT-qPCR quantification of IFNB, STAT1, and STAT2 expression in WT and OLFML3^−/− cells at 0, 12, and 24 h after RV-B14 infection at an MOI of 2. For c and d, gene expression is normalized to RPLP0 and significant difference was determined using two-way ANOVA with Sidak’s multiple comparisons test. Gene abbreviations are as follows: IFNB, interferon β; ISG15, IFN-stimulating genes 15; IFIT2, interferon induced protein with tetratricopeptide repeats 2; OAS2, 2′-5′-oligoadenylate synthetase 1; SOCS3, suppressor of cytokine signaling 3