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. 2021 Sep 25;10(10):1164. doi: 10.3390/antibiotics10101164

Figure 1.

Figure 1

MexAB-OprM is not involved in the PAO1-AzEvB8 mutant in vivo virulence. (A) Deletion of the mexAB genes abrogates aztreonam resistance in strain PAO1-AzEvB8. Aztreonam MICs were determined by gradient diffusion assays (n = 3 replicates/group; MIC: minimum inhibitory concentration). (B) mexAB deletion in PAO1-AzEvB8 mutant does not significantly improve mouse survival following acute lung infection. Log-rank test was used to compare survival curves. * p < 0.05 compared to WT PAO1; n = 10–15 mice/group. (C) Genome diagram showing coverage of sequencing reads aligning to the region spanning mexEF through xylZ. The 19,233 bp deleted region (indicated by the red arrow) in strains PAO1-AzEvB8 and PAO1-AzEvB8 ΔmexAB begins at the 3′ end of mexF and continues through the 5′ region of antC. Genome coverage plots generated from sequencing read alignments to the PAO1 reference genome are indicated in green.