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. 2021 Sep 25;10(10):1164. doi: 10.3390/antibiotics10101164

Figure 2.

Figure 2

MexEF-OprN mutation increases swarming motility and biosurfactant production. (A) Swarming is increased in the PAO1-AzEvB8 and ΔmexEF strains. Measurement of the swarming motility in different mexAB-oprM and mexEF-oprN mutants after 24 h, n = 6–7 replicates/group. (B) Representative images of swarming motility from panels (A,D). Scale bars indicate 10 mm. (C) Rhamnolipid gene expression is increased in the ΔmexEF strain. Rhamnolipid production measured by quantification of rhlA gene expression using a rhlA-gfp promoter reporter fusion to measure GFP fluorescence over time. Gene expression was calculated as area under the curve for each strain and compared to all other groups, n = 6–7 replicates/group. (D) Swarming is increased in mexE, mexF, and oprN PAO1 transposon mutant strains. Measurement of the swarming motility in different mexR and mexEF-oprN transposon mutants vs. a neutral Tn mutant control (PA3033) after 24 h, n = 5–12 replicates/group. For all panels, * p < 0.05, **** p < 0.0001, one-way ANOVA, followed by a Tukey’s multiple comparisons test.