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. 2021 Sep 25;10(10):1164. doi: 10.3390/antibiotics10101164

Figure 4.

Figure 4

Rhamnolipid gene expression is induced by the PaβN efflux pump inhibitor. (A) rhlA expression in WT PAO1 attB::rhlA-gfp treated with 0–25 μg/mL PAβN as measured by GFP fluorescence from the attB::rhlA-gfp promoter reporter fusion at 24 h (*** p < 0.0005, compared 0, 1, and 5 μg/mL, one-way Brown−Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparisons test, n= 6 replicates/group). (B–D) rhlA expression in PAO1 attB::rhlA-gfp (B), PAO1 ΔmexAB attB::rhlA-gfp (C), and PAO1 ΔmexEF attB::rhlA-gfp (D), treated with or without 25 μg/mL PAβN as measured by GFP fluorescence from the attB::rhlA-gfp promoter reporter fusion at 24 h. Gene expression was calculated as GFP fluorescence normalized to cell density (**** p < 0.0001; ns: not significant, p > 0.05, one-way Brown−Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparisons test, n = 6–12 replicates/group).