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. 2021 Oct 14;11(10):2962. doi: 10.3390/ani11102962

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Equine PBL transfer into NSG hosts. (A) Donor horse peripheral blood lymphocyte populations post-gradient centrifugation separation and prior to engraftment. (B–D) Percentages of equine CD4+, CD8+, CD21+ lymphocyte populations, respectively, in the peripheral blood of mouse recipients at day 7, 14, and 28 post-engraftment. (E) Percentages of equine CD4+, CD8+, CD21+ lymphocyte populations in the spleens of mouse recipients at 28 days post-engraftment. Splenic cells from the remaining 3 mouse hosts were cultured for 12 hours in the presence of PMA and ionomycin. Interferon gamma positive cells were assessed and compared with unstimulated cells (F).