Figure 4.

Quantitative gene expression of prolactin gene in pituitary after either bromocriptine or nano-bromocriptine administration. The qRT-PCR data were normalized relative to the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as endogenous control and calculated using the $2^{-\Delta \Delta \mathrm{CT} }$ method (n = 5). Different superscripts indicated significant differences at p < 0.05. Error bars indicate the standard error of the mean.