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PCR primers used in this study.
| Genes | Sequences (5′ to 3′) | Amplicon Size (bp) |
|---|---|---|
| tet(X6) | F: CGAGCTCATGACTTTACTAAAACATAAAAAAATTAC R: CCCAAGCTTTTATAGATTCATTAGTTTTTGGAAAGAA |
1149 |
| qPCR-tet(X6) | F: TGTCGTTGATTTTCTCCTG R: TTGATTCTGCCTGTGCTT |
332 |
| 16S rRNA | F: TTCGGGAACCGTGAGA R: CTGGCAACAAAGGATAAGG |
103 |
The underlined sequence represents restriction enzyme cutting site.
