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Alternative binding of Grb2-Gab1 and Grb2-Sos. (A) Sos-1 was immunoprecipitated (IP) from control or EGF-stimulated cell lysates (1 mg of total proteins) and immunoblotted (IB) with an anti-Grb2 antibody. The same membrane was stripped and reprobed by the anti-Sos antibody to determine the amount of Sos protein precipitated from each sample. (B) Immunoprecipitation was performed with an anti-Gab1 antibody, blotted with an anti-Grb2 antibody, and then reprobed with anti-Gab1.
