Table 1.
Molecular results from tissue and cytology cell sampler sampling methods using both the DNA Tissue Kit STM (QuickGene, Kurabo, Japan) and the DNeasyTM Blood and Tissue Kit (Qiagen, Inc., Valencia, CA, USA) on skin lesions from a bottlenose dolphin and an Atlantic spotted dolphin stranded on the Tenerife coasts, Canary Islands, Spain, 2021.
| Case | Lesion | Tissue | Cytology Cell Sampler | ||||
|---|---|---|---|---|---|---|---|
| QuickGene | QuickGene | Qiagen | |||||
| CePV | Ct | CePV | Ct | CePV | Ct | ||
| 1 | A | + | 23.65 | + | 25.46 | + | 20.85 |
| B | + | 31.80 | + | 27.93 | + | 26.70 | |
| 2 | A | + | 15.65 | + | 17.25 | + | 17.14 |
| B | + | 18.08 | + | 20.80 | + | 19.58 | |
| C | + | 16.42 | + | 19.04 | + | 15.17 | |
| D | + | 33.63 | + | 34.45 | + | 34.27 | |
| E | + | 25.02 | + | 33.55 | + | 34.74 | |
| F | + | 33.44 | + | 34.31 | + | 35.09 | |
| G | + | 31.79 | − | n/a | + | 37.10 | |
| H | + | 35.37 | − | n/a | + | 35.66 | |
| I | + | 28.49 | + | 33.33 | + | 31.97 | |
| J | − | n/a | + | 33.20 | + | 31.88 | |
Notes: CePV-1, cetacean poxvirus; Ct, cycle threshold; Qiagen, DNeasy Blood and Tissue Kit (Qiagen, Inc., Valencia, CA, USA); QuickGene, DNA Tissue Kit S (QuickGene, Kurabo, Japan); +, positive; −, negative; n/a, not applicable.