Figure 3.

Predicted plots (±95% confidence interval) for the different behaviours observed during euthanasia with CO₂ for the actual doses of the sedative tiletamine-zolazepam in C57BL/6 mice: (a) latency to ataxia from the onset of gas exposure (CO₂); (b) latency to recumbency from the onset of ataxia; (c) latency to laboured breathing from the onset of recumbency; (d) latency to last breath from the onset of laboured breathing. Mice were observed until last breath (1.67 ± 0.31 min; mean ± SD). Each dot represents an individual mouse.