(A). Effects of different irradiance ratios of aBL and aPDT in the dual-light aPDT system in one-day S. aureus biofilm daily at 200 J/cm2. One-day S. aureus biofilm, single dose, aBL vs. dual-light 1:3 aBL/aPDT ratio at 200 J/cm2, p = 0.0022; aBL vs. dual-light 1:1 aBL/aPDT ratio, p = 0.0022; aBL vs. dual-light 3:1 aBL/aPDT ratio, p = 0.0022; aPDT vs. dual-light 1:3 aBL/aPDT ratio, p = 0.0022; aPDT vs. dual-light 1:1 aBL/aPDT ratio, p = 0.0022; aPDT vs. dual-light 3:1 aBL/aPDT ratio, p = 0.0022. aBL vs. aPDT, p = ns; dual-light aPDT: 1:3 aBL/aPDT ratio vs. 1:1 aBL/aPDT ratio, p = ns; 1:3 aBL/aPDT ratio vs. 3:1 aBL/aPDT ratio, p = ns; 1:1 aBL/aPDT ratio vs. 3:1 aBL/aPDT ratio; p = ns. The y-axis displays the median of CFUs. Six assays were performed for each experiment, and the control biofilm was performed in three assays. The 95% confidence interval (CI) is presented by using t-bars; ns = non-significant. (B). A schematic representation of the experiment.