Table 5.

Dose response in vitro analysis.

	NKB			NKF
Mutation	WT	F251A	M289A	WT	F251A	M289A
EC50 (nM)	2.48 ± 0.05	X	X	65.22 ± 18.43	X	X
Min effective dose (nM)	0.316	X	X	0.316	X	X
Max effect (Luc fold activation)	3.36 ± 0.46	2.0 ± 0.32	1.71 ± 0.46	3.87 ± 0.38	1.99 ± 0.04	1.84 ± 0.17

EC50 (nM), minimal effective dose (nM) and maximal effect (Luc fold activation) for the native tilapia Tac3Ra and for the Tac3Ra mutants, F251A and M289A. EC50 values and minimal effective doses were calculated when maximal effect (Luc fold activation) was >2.0. (Data from Figure 4).